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3BN treatment setup and response in Arabidopsis col-0 (wild-type). (A) Three weeks old Arabidopsis (wild-type) plants were separately treated with different concentrations of 3BN in closed containers for 24 h. (B) The lesion phenotype was observed for 2.5 mM 3BN treatment (red arrows) and severe necrosis was observed for 3BN concentrations ≥ 5.0 mM. Photographs were taken 2 days post-3BN treatment.
