Figure 1.

Identification of GPLD1 as a candidate gene related to changes in Aβ production during neuronal differentiation of iPS cells. a, schematic representation of the experimental design. The iPS cell clones 201B7 and 253G4 were derived from healthy people with normal cognitive functions. The AD4F-1 clone was derived from a patient with sporadic AD. Three cultures derived from each of the iPS clones were subjected to neuronal differentiation and harvested for RNA preparation at days 38, 45, and 52. The amounts of secreted Aβ40 and Aβ42 increased during neuronal differentiation. The Aβ42/40 ratio was dramatically decreased at days 45 and 52 compared with that at day 38. We termed samples from day 38 as “before” and samples from days 45 and 52 as “after” according to the observed changes in the Aβ42/40 ratio. b, the GPLD1 expression levels in the three cultures derived from each of the original iPS cells (201B7, 253G4, and AD4F-1) during neuronal differentiation were compared before and after changes of the Aβ42/40 ratio. The average of the -fold increase in GPLD1 among the three lines is 1.42 (1.59-, 1.85-, and 1.29-fold for 201B7, 253G4, and AD4F-1, respectively). Error bars, S.D.