Figure 5.

CLK8 altered CLOCK protein levels in a time-dependent manner. Confluent U2OS cells were synchronized by 2-h treatment with dexamethasone (0.1 μm) and the medium was replaced with fresh medium containing CLK8 or DMSO. Cells were harvested at the indicated time points. Asterisk indicates nonspecific band. A, time-dependent analysis of core clock proteins by Western blotting. Data are mean ± S.E.; n = 3. B, qPCR analysis of the core clock genes in a time-dependent manner. Data are the mean ± S.E.; n = 3 independent experiments. ***, p < 0.001; **, p < 0.005; *, p < 0.05 versus the DMSO control by two-way ANOVA.