Table 1.

Potential targets of CLK8

Whole cell lysates of HEK293T cells overexpressing Clock and Bmal1 were incubated with 20 μm biotinylated CLK8. When needed, CLK8 was added as a competitor. Precipitated proteins in the pulldown assay were subjected to LC-MS/MS. The peptide spectrum matches and coverage of the main target, CLOCK, were considered as the threshold in data analysis. Proteins with peptide spectrum matches and coverage lower than threshold were eliminated.

	Protein	Peptide spectrum matches
		Competitor (μm)
		0	200
CLOCK	Circadian locomoter output cycles protein kaput	42	–
BMAL1	Aryl hydrocarbon receptor nuclear translocator-like protein 1	56	46
NAV2	Neuron navigator 2	263	188