Figure 1.

Integration of unprocessed prespacer recovered by DnaQ and ExoT. A, schematic of plasmid integration assay. SC, supercoiled plasmid; OC, open circle plasmid. Repeats are gray, spacers are blue and yellow, and the leader sequence is pink. The entire array is not represented. B, agarose gels of integration assays using a processed prespacer (PP) or unprocessed prespacer (UP) in the absence or presence of 3′–5′ exonucleases, alongside linearized (HindIII-treated) plasmid, a control reaction lacking prespacer, and untreated plasmid.