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. 2019 Dec 30;295(11):3403–3414. doi: 10.1074/jbc.RA119.012196

Figure 5.

Figure 5.

The presence of PAM blocks spacer-side integration of the non-PAM strand. A, schematic of oligonucleotide target integration assay with asymmetric prespacer, showing expected half-site products of integration of the non-PAM strand in either the correct (green checkmark) or incorrect (red X) orientation. B, denaturing gels of time courses of the oligonucleotide integration assay using three different prespacers (PS), shown by schematics above each gel. The reaction is started with the addition of the CRISPR target. 0, mock reaction without any target. M, marker lane; radiolabels are indicated by a star. Schematics of expected products are to the right of the gel images.