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The insensitive cell line HCC15 does not express PDE3A protein. A (left), PDE3A protein expression was analyzed by immunoblotting in cancer cell lines. Right, PDE3A or GAPDH mRNA expression was analyzed by quantitative PCR. mRNA expression displayed as log2(relative gene expression) values. B, ectopic expression of PDE3A confers DNMDP sensitivity in the HCC15 cells, assayed by a 72-h CellTiter-Glo assay. Ectopic PDE3A expression was confirmed by immunoblotting. NT, no transduction.
