Figure 5.

DENND1 family proteins are not required for PODXL trafficking under 2D culture conditions. Parental, Rab35-KO, DENND1A-KO, DENND1B-KO, and DENND1C-KO cells were plated on glass-bottom dishes and fixed at 3 h after plating. The cells were stained for PODXL (green), actin (red), and DAPI (blue), followed by counting of cells with co-localized PODXL and actin. The arrowheads show PODXL co-localized with actin. Scale bars, 10 μm. The graph shows the means and S.E. (error bars) of three independent experiments. *, p < 0.05; NS, not significant (Dunnett's test).