Figure 7.
Mycothiol protects Cys-127 in the presence of hydrogen peroxide. A, Cd-MsrB is not recycled via the mycothiol reduction pathway. Progress curves show no consumption of NADPH when Cd-MsrB is coupled to the MSH/Mrx1/Mtr pathway after adding MetSO as a substrate. B, in the presence of mycothiol and H2O2, Cd-MsrB gets S-mycothiolated and becomes substrate of the MSH/Mrx1/Mtr reduction pathway. The rate of demycothiolation is 17.0 ± 0.4 milli-absorbance units/min. Data are presented as a mean ± S.D. of three independent technical repeats and the graphs were generated using Prism8.