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. 2020 Jan 16;295(11):3601–3613. doi: 10.1074/jbc.RA119.010489

Figure 1.

Figure 1.

Mice deleted for Hv1 display hyperglycemia and impaired glucose tolerance because of reduced insulin secretion. A, body weight change of Hv1−/−, Hv1+/−, and WT mice (n = 24/genotype). Data are means ± S.D. B and C, basal blood glucose (B) and corresponding serum insulin (C) concentrations after fasting for 6 h in 4-month-old Hv1−/−, Hv1+/−, and WT mice (n = 13 for Hv1−/− and Hv1+/− and n = 10 for WT). Data are means ± S.D. ***, p < 0.001; Hv1−/− or Hv1+/− versus WT. D–F, HOMA parameters calculated from basal blood glucose (B) and corresponding serum insulin (C) concentrations. Data are means ± S.D. *, p < 0.05; **, p < 0.001; Hv1−/− or Hv1+/− versus WT. HOMA-IR was calculated as follows: fasting glucose (millimoles per liter) × fasting insulin (milliunits per liter) / 22.5. Homeostasis model assessment insulin sensitivity is the reciprocal of HOMA-IR. Homeostasis model assessmen islet β cell insulin secretory function was calculated as follows: 20 × fasting insulin (milliunits per liter) / (fasting glucose (millimoles per liter) − 3.6). G and H, Blood glucose levels measured in whole blood following i.p. injection of glucose (2 g/kg of body weight) in Hv1−/−, Hv1+/−, and WT mice (G) and AUC (H) (n = 12/genotype). Data are means ± S.E. ***, p < 0.001; Hv1−/− versus WT. I and J, insulin concentrations measured in sera of Hv1−/−, Hv1+/−, and WT mice following i.p. glucose challenge (2 g/kg of body weight) (I) and AUC (J) (n = 8/genotype). Data are means ± S.E. **, p < 0.01; ***, p < 0.001; Hv1−/− or Hv1+/− versus WT. K, blood glucose levels measured in whole blood following i.p. insulin injection (1 unit/kg of body weight) in Hv1−/−, Hv1+/−, and WT mice (n = 12/genotype). Data are means ± S.E. *, p < 0.05; Hv1−/− versus WT.