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. 2020 Jan 16;295(11):3601–3613. doi: 10.1074/jbc.RA119.010489

Figure 3.

Figure 3.

Knockout of Hv1 inhibits insulin secretion in isolated islets. A, glucose- and KCl-induced insulin secretion from isolated islets of Hv1−/−, Hv1+/−, and WT mice (n = 8/genotype). Data are means ± S.E. ***, p < 0.001, Hv1+/− versus corresponding WT; ###, p < 0.001, Hv1−/− versus corresponding WT; &&&, p < 0.001, glucose (Glc) or KCl versus basal for WT. Basal, 2.8 mm glucose; glucose, 16.7 mm glucose; KCl, 60 mm KCl. B–D, time-dependent GSIS stimulated by 16.7 mm glucose (B), AUC (C), and amounts of first- and second-phase insulin secretion (D) from isolated islets of WT and KO mice (n = 6/genotype). Data are means ± S.E. *, p < 0.05; **, p < 0.01; versus corresponding WT. E, perifusion assays for the kinetics of insulin secretion in isolated islets of KO and WT mice (n = 8/genotype). The arrow indicates the time of 16.7 mm glucose addition. F and G, insulin (F) and proinsulin (G) content under basal conditions in isolated islets from Hv1−/−, Hv1+/−, and WT mice (n = 8/genotype). Data are means ± S.E. **, p < 0.01; ***, p < 0.001; Hv1−/− versus WT. H, ratio of insulin to proinsulin content of isolated islets of Hv1−/−, Hv1+/−, and WT mice under basal conditions (n = 8/genotype). Data are means ± S.E. NS, no significance, versus WT. I, proinsulin secretion at 16.7 mm glucose from isolated islets of Hv1−/−, Hv1+/−, and WT mice (n = 8/genotype). Data are means ± S.E. ***, p < 0.001; Hv1−/− or Hv1+/− versus WT. J and K, ratio of insulin to proinsulin secretion (J) and of insulin secretion to insulin content (K) of isolated islets of Hv1−/−, Hv1+/−, and WT mice at 16.7 mm glucose (n = 8/genotype). Data are means ± S.E. *, p < 0.05; ***, p < 0.001; Hv1−/− or Hv1+/− versus WT.