Skip to main content
. 2020 Jan 16;295(11):3601–3613. doi: 10.1074/jbc.RA119.010489

Figure 7.

Figure 7.

Down-regulation of Hv1 in islets of the STZ-induced diabetes model and glucose-induced dysfunctional β-cells. A, basal blood glucose concentrations after fasting for 6 h in STZ-treated KO and WT mice (n = 23 for WT; n = 27 for KO). Data are mean ± S.E. *, p < 0.05; KO versus WT. B, blood glucose levels measured in whole blood following i.p. injection of glucose (2 g/kg of body weight) in STZ-treated KO and WT mice (n = 12 for WT; n = 14 for KO). Data are means ± S.E. *, p < 0.05; KO versus WT. C, insulin contents under basal conditions (2.8 mm glucose) in islets isolated from control and STZ-treated WT mice (n = 10/genotype). Data are means ± S.E. ***, p < 0.001; STZ versus control. D, 16.7 mm glucose-induced insulin secretion of islets isolated from control and STZ-treated WT mice (n = 8/genotype). Data are means ± S.E. ***, p < 0.001; STZ versus control. E, immunohistochemical analysis of pancreas from mice treated with STZ (b and d) or saline (a and c, control) using anti-Hv1 and anti-insulin monoclonal antibodies and representative images of anti-Hv1 (a and b) and anti-insulin (c and d) antibody-stained pancreatic sections. Scale bar = 50 μm. Mean optical density of Hv1- and insulin-positive areas of islets of control and STZ-treated WT mice (e, Hv1, top; f, insulin, bottom). Twenty to thirty sections per pancreas were analyzed (n = 8/condition). Data are means ± S.E. *, p < 0.05; ***, p < 0.001; STZ versus control. Hv1 is down-regulated in pancreatic islets in STZ-induced diabetic mice. F, Glucose-induced insulin secretion from INS-1 (832/13) cells chronically incubated at 11 (control) and 25 mm glucose for 48 h. Data are means ± S.E. (n = 8 per condition). ***, p < 0.001, vs. control. G, Immunofluorescence analysis of INS-1 (832/13) cells chronically incubated at 11 (control) and 25 mm glucose for 48 h using anti-Hv1 monoclonal antibody. Representative images of anti-Hv1 monoclonal antibody-stained INS-1 (832/13) cells (a and c) and DAPI stain to visualize the nuclei (b and d). Scale bar, 20 μm. Mean fluorescence intensity of Hv1-positive area of the cells (e), expressed as an average of five experiments (Five different perspectives were counted per experiment. 50–150 cells per perspective). Data are means ± S.E. ***, p < 0.001, vs. control. Hv1 is also obviously down-regulated in dysfunctional INS-1 (832/13) cells.