Figure 2.

MUTYH expression alters MEF survival to MNNG. A, normalized survival versus control condition 6–7 days after treating with low MNNG (67 μm) in Mutyh^−/−, wildtype (WT), and Mutyh^−/− MEFs stable cell lines expressing recombinant human MUTYH, classified as low MUTYH (expression) and high MUTYH (expression) based on Western blotting. Data are from at least four biological replicates. **, p < 0.005, t test, significant with Bonferroni correction for multiple comparisons. B, Western blotting of representative Mutyh^−/− MEF stable cell lines transfected with the pcDNA3.1 human MUTYH construct after G418 antibiotic selection. HEK-293 cell lysate is shown as a MUTYH-positive control. Clones A and C were categorized as high MUTYH expression. Clones B and D were categorized as low MUTYH expression. The figure is composed of two separate Western blottings using the same antibodies and conditions as detailed under “Experimental procedures.”