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. 2020 Mar 16;40(7):e00228-19. doi: 10.1128/MCB.00228-19

FIG 7.

FIG 7

Stage VII round spermatid block in the Zfp628−/− seminiferous epithelium. Shown is comparative testis histology between Zfp628+/− (A to C) and Zfp628−/− (D to F) testis sections during 3 later stages of the seminiferous epithelium using PAS staining. (A and D) Insets of round spermatids at stage VI show improper acrosomal cap development in Zfp628−/− testis. (B and E) At stage VII/VIII, acrosomal granules (black arrows) are still present in Zfp628−/− testis, while acrosomal caps (white arrows) are properly formed in Zfp628+/− testis. The red circle highlights normal elongated spermatids in the Zfp628+/− testis, whereas the black circle indicates the sloughing of large multinucleated round spermatids in the Zfp628−/− testis that lack these elongated spermatids. (C and F) A stage XII tubule section shows a few round spermatids with acrosomal cap (white arrows) but no elongated spermatids (red arrows) or sperm at this stage of the seminiferous epithelium.