TABLE 2.
RT-qPCR and genotyping primers
| Protein or construct | Forward primera | Reverse primera |
|---|---|---|
| RT-qPCR primers | ||
| ZFP628 | ACCGAGGGAACTGGGGAAAA | GAGATGACCACCTGAAGGACT |
| GAPDH | AGGTCGGTGTGAACGGATTTG | TGTAGACCATGTAGTTGAGGTCA |
| Taf7l | AGCCCCCGTTATTCCTGAAG | CCTCGGGTGTCACAAGTGT |
| Taf7 | AACAAAGACGATGCGCCTCAT | CAGGTTGACATGCCCAGACT |
| Trf2 | TTTGGTGCCAGACGTTTAGC | GCCACAGCCTTTACATTGGG |
| TBP | ATGATGCCTTACGGCACAGG | GTTGCTGAGATGTTGATTGCTG |
| Taf4b | TTGCAGCTATTGGACCAAGGA | GTGGCTGTTAGGCTGGAAGT |
| Taf4 | GAATGGTCCTCGTGCGGAG | CCGTGCAATAATAGGTGTTCCT |
| Stra8 | TTTGACGTGGCAAGTTTCCTG | TAACACAGCCAAGGCTTTTGA |
| Plzf | CTGGGACTTTGTGCGATGTG | CGGTGGAAGAGGATCTCAAACA |
| Gfra1 | CACTCCTGGATTTGCTGATGT | AGTGTGCGGTACTTGGTGC |
| Tnp1 | ACCAGCCGCAAGCTAAAGAC | GCTTCCACCTCTCTTGACGC |
| Tnp2 | TCACACCAGTAACCAGTGCAA | CCCTGAGCTACGCCTCTTAG |
| Prm1 | CCGTCGCAGACGAAGATGTC | CACCTTATGGTGTATGAGCGG |
| Prm2 | ATGGTTCGCTACCGAATGAGG | CTCCGCCTTCTGCATGACC |
| Ccna1 | TGATGCTTGTCAAATGCTCAGC | AGGTCCTCCTGTACTGCTCAT |
| Miwi | GACTCGGAATGGGGAACACG | GGTTTGGAATATCAATCGGGTCA |
| Mov10l1 | CGCTGTGACGAGTACAGTG | CTGACAACCCTTTGCTAGAGTTT |
| CREM | ATGGTTTCTGTAGCTGGATCAGG | GATGTGGTGTCTGAATAACTCCC |
| Tdrd5 | GGAGCCATAAGGTCCGAAACT | AGAACGGGAGACAATGCCAAA |
| Flh5 | TTGTGAGCAGTGTAAAGAACCAA | ACCAAAGAGTGATGGCATTTGTT |
| Zfp628 genotyping primer construct 628sgRNA-F | AGCCTTCAAGGGTTCCTCAG | AGCAGGTTGGAGGAGTGTGT |
a
All primers are shown in the 5′-to-3′ direction. The sequence for the Zpf628 stop codon cassette is as follows: TGGTCTGGCCTTCAAGTGGTCATCCCATTACCAATACCACTTGAGACAGCATACCGGTGAGTCATGGCGTTTAAACCT*TAAT*TAAGCTGTTGTAGGCGGCCCTACCCGTGCCCTGACTGCCCCAAGGCCTTCAAGAACTCCTCCAGCTTGCGGCG, where bold text indicates the transgene insertion sequence and underlined text indicates the stop codon sequence.