Table 3. Proteomic data of the differentially expressed proteins identified in the spermatozoa samples of fertile men (control) and men with non-seminoma testicular cancer (NSTC) before cancer therapy, which were selected for validation by Western blot.
| Protein | Abundance | NSAF ratio | Expression profile | p-value | |
|---|---|---|---|---|---|
| Control | NSTC | ||||
| ACR | High | High | 0.60 | Underexpressed in NSTC | 0.0005 |
| ANXA2 | Medium | High | 2.05 | Overexpressed in NSTC | 0.0003 |
| ATP1A4 | Medium | Low | 0.47 | Underexpressed in NSTC | 0.0009 |
| NDUFS1 | Medium | Very low | 0.40 | Underexpressed in NSTC | 0.0156 |
| UQCRC2 | High | Medium | 0.61 | Underexpressed in NSTC | 0.0011 |
NSAF: normalized spectral abundance factor, ACR: acrosin precursor, ANXA2: Annexin A2, ATP1A4: sodium/potassium-transporting ATPase subunit alpha-4, NDUFS1: NADH-ubiquinone oxidoreductase 75 kDa subunit, UQCRC2: cytochrome b-c1 complex subunit 2.