Figure 5.

Effect of phytohormones in N starvation responses on roots. (A) Relative levels of Indol acetic acid (IAA), Abscisic acid (ABA), Jasmonic acid (JA), Jasmonic isolecuine (JAIle) and Salicylic acid (SA) in root or shoot of DGR seedlings that were cultivated in MS1/2 with high N (+N; 2500 µM) or with low N medium (50N; 50 µM) during 8 days. *, p < 0.05; **, p < 0.01; ***, p < 0.001 by t-Test comparing between high and low N. (B,C) Percentage of root growth (main root or main + LR length) of seedlings grown for 5 days in MS1/2 medium with high (2500 µM) (B) or low (50 µM) N (C) and then 5 days to a similar medium containing 0 or 50 µM of SA. *, p < 0.05; **, p < 0.01; ***, p < 0.001 by t-Test comparing to the control. (D,E) NO₃⁻ levels in control seedlings grown in MS1/2 medium containing high N (2500 µM) (D) or low N (50 µM) (E) during 5 days and then transferred to a similar medium containing 0 or 50 µM of SA for 5 extra days. *, p < 0.05; **, p < 0.01; ***, p < 0.001 by t-Test comparing to the control in each condition. (F) Relative fresh weight (FW) of seedlings treated with or without SA that were grown as in (D). (G) Relative fresh weight (FW) of seedlings grown with or without N that were grown as in (E). *, p < 0.05; **, p < 0.01; ***, p < 0.001 by t-Test comparing to the control in each condition. Error bars correspond to S.E.