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. 2020 Feb 15;9(2):249. doi: 10.3390/plants9020249

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Analysis of differences in protoxylem maturation in 10-day-old leaf-wounded (LW) WT and mutant (Atcuaoβ.1 and Atcuaoβ.2) seedlings, grown in medium with or without 100 µM Put. Distances from the apical meristem of the protoxylem position (defined by the position of the first protoxylem cell with fully developed secondary cell wall thickenings) are reported. These experiments were repeated at least three times with ten seedlings analyzed each time (mean values ± SD; n = 30). The statistical significance levels (p-values) were evaluated with one-way analysis of variance (ANOVA) followed by Sidak’s multiple comparison test (****, p ≤ 0.0001; ***, p ≤ 0.001). Insignificant differences are not indicated.