Table 2.
Sensitivity of the PCR (C45 primers), qPCR (C45 primers), and LAMP (group 5 primers) tests in seed matrix contaminated with a Triticum lineage P. oryzae strain (BL0092).
| BL0092 | Number of Contaminated Grains Per Lot | C45 PCR | C45 qPCR | Group 5 LAMP | ||
|---|---|---|---|---|---|---|
| Positive Replicates/Total Replicates | Positive Replicates/Total Replicates | Mean Ct ± SD | Positive Replicates/Total Replicates | min ± SD | ||
| 0 grain | 0/9 | 0/9 | >40 | 0/4 | - | |
| 0 h | 1 grain | 1/9 | 3/9 | 37.21 ± 0.74 | 0/4 | - |
| 2 grains | 2/9 | 1/9 | 37.31 | 0/4 | - | |
| 5 grains | 3/9 | 4/9 | 37.66 ± 1.45 | 0/4 | - | |
| 10 grains | 6/9 | 7/9 | 37.16 ± 0.88 | 0/4 | - | |
| 15 grains | 9/9 | 6/9 | 36.74 ± 1.17 | 1/4 | 13:74 | |
| 50 grains | 9/9 | 9/9 | 35.36 ± 0.60 | NT | NT | |
| 0 grain | 0/9 | 0/9 | >40 | 0/4 | - | |
| 72 h | 1 grain | 9/9 | 9/9 | 33.83 ± 0.61 | 2/4 | 12:97 ± 10:80 |
| 2 grains | 9/9 | 9/9 | 30.97 ± 0.41 | 4/4 | 4:66 ± 0:55 | |
| 5 grains | 9/9 | 9/9 | 29.00 ± 0.32 | 4/4 | 4:24 ± 0:09 | |
| 10 grains | 9/9 | 9/9 | 29.32 ± 0.25 | 4/4 | 4:22 ± 0:18 | |
| 15 grains | 9/9 | 9/9 | 28.56 ± 0.25 | 4/4 | 4:13 ± 0:24 | |
| 50 grains | 9/9 | 9/9 | 26.47 ± 0.32 | NT | NT | |