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. 2020 Feb 4;9(2):189. doi: 10.3390/plants9020189

Table 3.

Overview of sequencing read counts in four sRNA libraries. TCG, constructed from DBA Aurora CG seedlings. TWH, constructed from DBA Aurora WH seedlings. SCG, constructed from L6 CG seedlings. SWH, constructed from L6 WH seedlings. Clean reads may not be equal to raw reads minus all the filter type reads, as there could be overlapped sequences between mRNA, Rfam and Repeats filters.

Read Type TCG TWH SCG SWH
Total Reads Unique Reads Total Reads Unique Reads Total Reads Unique Reads Total Reads Unique Reads
Raw reads 20,042,833 8,293,425 20,900,186 8,593,357 24,262,343 8,865,724 18,786,468 7,221,909
3ADT & Length filter 6,000,364 1,418,893 5,651,835 1,475,235 7,669,010 1,832,961 6,589,933 1,676,502
Junk reads 92,538 68,473 101,066 73,192 111,339 79,088 76,371 56,322
Rfam - rRNA 215,716 5440 228,715 5378 308,312 6809 266,089 7444
Rfam - tRNA 230,895 2119 215,340 2207 248,091 2853 310,937 3964
Rfam - snoRNA 9600 393 9813 396 10,886 363 8009 304
Rfam - snRNA 2364 181 2581 194 3250 264 3580 215
Other Rfam RNA 13,680 733 14,276 706 19,445 886 17,165 893
mRNA 878,039 23,905 915,468 25,170 1,090,076 26,584 798,629 20,880
Repeats 6104 186 6073 193 9312 265 7704 243
Clean reads 12,631,656 6,773,722 13,795,248 7,011,288 14,830,872 6,916,493 10,781,672 5,456,127