Table 3.
Compositions of the Box–Behnken experimental design and their observed responses.
| Standard Run | Independent Variables/Factors | Z-Average (nm) |
EE (%) |
||
|---|---|---|---|---|---|
| MEL (mg/mL) |
HSA (mg/mL) |
Tween 80 (mg/mL) |
|||
| 1 | 1.0 | 5.0 | 3.0 | 371.5 ± 3.8 | 68.4 ± 1.7 |
| 2 | 3.0 | 5.0 | 3.0 | 168.3 ± 4.1 | 72.4 ± 3.1 |
| 3 | 1.0 | 15.0 | 3.0 | 282.4 ± 6.2 | 53.5 ± 2.4 |
| 4 | 3.0 | 15.0 | 3.0 | 285.3 ± 7.5 | 68.1 ± 1.9 |
| 5 | 1.0 | 10.0 | 0 | 481.3 ± 8.1 | 71.6 ± 1.2 |
| 6 | 3.0 | 10.0 | 0 | 1198 ± 13.9 | 34.6 ± 3.5 |
| 7 | 1.0 | 10.0 | 6.0 | 220.6 ± 6.8 | 56.9 ± 0.9 |
| 8 | 3.0 | 10.0 | 6.0 | 246.8 ± 3.9 | 71.5 ± 1.4 |
| 9 | 2.0 | 5.0 | 0 | 423.4 ± 5.5 | 61.3 ± 2.3 |
| 10 | 2.0 | 15.0 | 0 | 663.8 ± 9.1 | 36.7 ± 3.2 |
| 11 | 2.0 | 5.0 | 6.0 | 102 ± 6 | 72.6 ± 1.8 |
| 12 | 2.0 | 15.0 | 6.0 | 308.2 ± 7.7 | 36 ± 3.7 |
| 13 | 2.0 | 10.0 | 3.0 | 175.6 ± 6.8 | 79.1 ± 2 |
| 14 | 2.0 | 10.0 | 3.0 | 176.1 ± 2.7 | 80 ± 1.8 |
| 15 | 2.0 | 10.0 | 3.0 | 175.9 ± 4.2 | 79.2 ± 2.1 |