Table 1.

Parameters of the UPLC–MS/MS system for analysis of nilotinib.

Column	BEH C18 (1.7 µm, 2.1 × 50 mm)
Flow rate	0.3 mL/min
Injection volume	5 µL
Column oven	40 °C
Sample oven	4 °C
Mobile phase A (MPA)	0.1% formic acid
Mobile phase B (MPB)	Acetonitrile
Elution condition	0~0.3 min, 90% MPA + 10% MPB
	0.3~1.6 min, 20% MPA + 80% MPB
	2~3 min, 90% MPA + 10% MPB
Capillary voltage	3 kV
Cone voltage	48 V
Desolvation temperature	350 °C
Desolvation gas flow	650 L/h
Collision gas flow	50 L/h