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. 2020 Apr;373(1):72–80. doi: 10.1124/jpet.119.264010

Fig. 2.

Fig. 2.

The effects of early, repeated intraperitoneal administration of retigabine on the recovery of locomotor function after SCI. (A) BBB scoring was performed −1, 1, 2, 3, 7, 14, 21, 28, 35, and 42 days after contusion. SCI animals received either 3 mg/kg retigabine (i.p., twice daily) or identical vehicle 3 hours after contusion for 10 consecutive days. Repeated measures two-way ANOVA followed by Sidak’s multiple comparison test. Treatment F(1, 26) = 0.1714, P = 0.6822; time F(9, 234) = 445.7, P < 0.0001; interaction F(9, 234) = 0.4473, P = 0.9081. Baseline, P > 0.999; 1 day, P > 0.999; 2 days, P > 0.999; 3 days, P > 0.999; 7 days, P = 0.9992; 14 days, P = 0.7635; 21 days, P > 0.999; 28 days, P > 0.999; 35 days, P > 0.999; 42 days, P > 0.999. (B) BBB scoring was performed −1, 1, 2, 3, 7, 14, 21, 28, 35, and 42 days after contusion. SCI animals received either 10 mg/kg retigabine (i.p., twice daily) or identical vehicle 3 hours after contusion for 10 consecutive days. Repeated measures two-way ANOVA followed by Sidak’s multiple comparison test. Treatment F(1, 18) = 0.3431, P = 0.5653; time F(9, 162) = 252.4, P < 0.0001; interaction F(9, 162) = 0.6304, P = 0.7699. Baseline, P > 0.999; 1 day, P > 0.999; 2 days, P > 0.999; 3 days, P > 0.999; 7 days, P = 0.6373; 14 days, P > 0.999; 21 days, P > 0.999; 28 days, P > 0.999; 35 days, P = 0.9471; 42 days, P > 0.999. (C) Horizontal ladder test was performed −1, 14, 21, 28, 35, and 42 days after contusion. SCI animals received either 3 mg/kg retigabine (i.p., twice daily) or identical vehicle 3 hours after contusion for 10 consecutive days. Repeated measures two-way ANOVA followed by Sidak’s multiple comparison test. Treatment F(1, 16) = 4.432, P = 0.0516; time F(5, 80) = 150.6, P < 0.0001; interaction F(5, 80) = 2.545, P = 0.035. Baseline, P > 0.999; 14 days, P = 0.8821; 21 days, P = 0.9904; 28 days, P = 0.9994; 35 days, P = 0.0133; 42 days, P = 0.0333. (D) Horizontal ladder test was performed −1, 14, 21, 28, 35, and 42 days after contusion. SCI animals received either 10 mg/kg retigabine (i.p., twice daily) or identical vehicle 3 hours after contusion for 10 consecutive days. Repeated measures two-way ANOVA followed by Sidak’s multiple comparison test. Treatment F(1, 13) = 7.821, P = 0.0151; time F(5, 65) = 145.9, P < 0.0001; interaction F(5, 65) = 2.352, P = 0.0503. Baseline, P > 0.999; 14 days, P = 0.1383; 21 days, P > 0.9999; 28 days, P > 0.9999; 35 days, P = 0.0057; 42 days, P = 0.1423. *P < 0.05.