Fig. 5.

Observed correlations between transporter protein abundance and relative mRNA expression levels. In (A), the x-axis represents mRNA expression levels of transporters normalized to mRNA expression levels of GAPDH, and the y-axis represents protein abundance. In (B), the x-axis represents mRNA expression levels of transporters normalized to mRNA expression levels of villin 1, and the y-axis represents protein abundance. Correlation analysis was assessed using Spearman rank-order correlation (Rs) test with t-distribution of the P value. Linear regression analysis was carried out to assess the linearity of relationships and scatter of the data (R²). Correlations were deemed strong when the Rs values were greater than 0.60, P values were less than 0.05, and the data points demonstrated limited scatter (R² > 0.30). Rs values between 0.5 and 0.6 with a P value less than 0.05 were considered to represent moderate correlation. Clear circles with a green border correspond to ileal samples, and clear circles with a red border correspond to jejunal samples.