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Protein release analysis. Release of total proteins from Alg/PL and Alg/SS/PL biomembranes at different time incubation in physiological solution at 37 °C and 5% CO2. The total protein content was determined with Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA) in the incubation media collected at different times. The experiments were performed in triplicate on three different batches of biomembranes.
