Table 1.
Physicochemical properties of Poly(D,L-Lactide-co-Glycolic Acid) (PLGA) nanoparticles (NPs). Determination of retinoic acid (RA) and oxaliplatin (OXA) content, size distribution, and zeta-potential of PLGA NPs. Particles were characterized by Dynamic Light Scattering (DLS) and zeta-potential measurements. Particle size data represent the mean value ± standard deviation (SD) of dynamic light scattering data. Zeta-potential data represent the mean value ± SD of 10 readings. OXA and RA contents of PLGA NPs were determined by particle digestion and measured by reversed-phase high-performance liquid chromatography (RP-HPLC) analysis.
| Samples | Loading Oxaliplatin Efficiency (µg/mg NPs) |
Loading Retinoic Acid Efficiency (µg/mg NPs) |
Size ± SD (nm) |
PDI (Polydispersity Index) |
Zeta Potential ± SD (mV) |
|---|---|---|---|---|---|
| NPs 1 (OXA, RA)-CHO | 44 | 40 | 801.7 ± 165.4 | 0.598 | −21.4 ± 8.4 |
| NPs 2 (OXA)-CHO | 48 | -- | 678.3 ± 118.5 | 0.694 | −25.8 ± 15.9 |
| NPs 3 (RA)-CHO | 46 | 539.8 ± 87.6 | 0.438 | −28.5 ± 16.1 | |
| NPs 4 (empty)-CHO | -- | -- | 443.1 ± 27.1 | 0.253 | −23.6 ± 9.13 |
| NPs 5 (empty) | -- | -- | 496.7 ± 35.35 | 0.255 | −28.8 ± 8.6 |
| NPs 6 (OXA) | 55 | -- | 391.5 ± 60.53 | 0.182 | −29.6 ± 9.9 |
| NPs 7 (OXA, RA) | 46 | 44 | 505.6 ± 64.30 | 0.199 | −27.6 ± 42.1 |
| NPs 8 (RA) | -- | 50 | 514.8 ± 106.7 | 0.136 | −25.7 ± 10.8 |