Figure 6.

Serpina3c Overexpression Increases Adipocyte Differentiation

3T3-L1 cells were transfected with a control (pcDNA 3.0) or Serpina3c overexpression vector and induced to differentiate.

(A) Overexpressed Serpina3c was confirmed 2 days after transfection by detection of the FLAG tag from whole-cell lysates. Proteins collected at the indicated times after induction were used to assay the levels of C/EBPα and PPARγ by western blot (GAPDH was used as loading control).

(B) Oil Red O staining on day 5.

(C) Serpina3c expression by quantitative real-time PCR in epididymal WAT tissues from C57BL/6 male mice fed a chow diet (CD) or a high-fat diet (HFD) for 8 or 16 weeks. WAT, white adipose tissue. Data are represented as mean ± SD. p Values less than 0.05 were considered significant, with ∗p < 0.05, ∗∗p < 0.01 as determined by Student's t test.

(D) SERPINA3 gene expression levels in adipose stem cells or preadipocytes from obese and lean individuals, using publicly available repository data (Gene Expression Omnibus accessions GDS5056 and GDS1480). Data are represented as mean ± SD. p Values less than 0.05 were considered significant, with ∗p < 0.05, ∗∗p < 0.01 as determined by Student's t test.