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. Author manuscript; available in PMC: 2021 Feb 3.
Published in final edited form as: Curr Biol. 2020 Jan 2;30(3):408–420.e5. doi: 10.1016/j.cub.2019.11.064

Figure 4. Initial steps of DSB repair take place normally on autosomes of MSCI defective mutants.

Figure 4.

(A, B, E, F) Chromosome spreads of wild-type (WT) littermate control and H2ax-Y142A (A, B) or Mdc1KO (E, F) mid pachytene spermatocytes immunostained with antibodies raised against SYCP3 (A, B, E, F), HIT (A, B, E, F), RAD51 (A, E), or MLH3 (B, F). Sex chromosomes in dashed squares are magnified in the panels to the right (A, E). Autosomes in dashed squares are magnified in the panels to the right, and the dashed circles indicate the sex chromosomes (B, F). Dot plots indicate the numbers of autosome RAD51 foci (A, E), sex chromosome RAD51 foci (A, E), or MLH3 foci (B, F) per mid pachytene (HIT-positive) spermatocyte, shown as mean ± s.e.m. for 3 independent H2ax-Y142A littermate pairs (A, B) and 3 independent Mdc1KO littermate pairs (E, F). Total numbers of analyzed nuclei are indicated in the panels. n.s.: not significant; **** p < 0.0001, unpaired t tests. XY chr.: XY chromosomes. Scale bars: 10 μm.

(C) Model of the MSCI checkpoint and its relationship to meiotic recombination.

(D) DAPI counterstaining of a 3D slide (see STAR METHODS). The dashed circle indicates the XY body. Scale bar: 10 μm.

See also Figures S3 and S4.