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. 2020 Jan 29;9(2):162. doi: 10.3390/plants9020162

Figure 1.

Figure 1

Structure of the expression vector pEff_Flg4M2eHA2-1. RDRP, RNA-dependent RNA polymerase of PVX; Sgp1, the first promoter of the subgenomic RNA of PXV; AMV, the leader sequence of RNA 4 of alfalfa mosaic virus; 6his, sequence that encodes the N-terminal 6-histidine tag; Flg, S. typhimurium flagellin; HA2, sequence of the HA fragment (76–130 a.a.); 4M2e, the sequence of four tandem copies of M2e peptide arranged as M2eh-M2es-M2eh-M2es; 35S, promoter of the cauliflower mosaic virus RNA; NosT, terminator of the nopaline synthase gene from Agrobacterium tumefaciens; P24, gene of silencing suppressor from grapevine leafroll-associated virus-2; LB and RB are the left and right borders of T-DNA.