Figure 1.

Wild type (WT), lacs2-3, and LACS2-OEs leaves showed changed hydrophobicities upon submergence. The hydrophobic cuticular wax of WT, lacs2-3, and LACS2-OE lines were detected by water droplets under normal growth (Air) and submergence (Submergence) conditions. Hydrophobicity of the leaf cuticular wax was assessed using the contact angle of small droplets of water, and contact angles <90° indicate a hydrophilic surface, whereas those of >90° indicate a hydrophobic surface. The experiments have been biologically repeated three times and similar results were obtained. For each experiment, at least six leaves were used per genotype. Data are average values ± SD (n = 3) calculated from three independent experiments.