Figure 4.

LACS2 regulated cuticle permeability in response to submergence treatment. (A) Cuticle permeability analysis in the leaves of four-week-old WT, lacs2-3, and LACS-OEs under normal growth (air) and submergence (submergence) conditions. The leaves were collected before and three days after treatment and stained with 0.05% toluidine blue. (B, C and D) Relative rates of chlorophyll leaching (B), water loss (C), and ion leakage (D) showing the cuticle permeability of four-week-old wild type (WT), lacs2-3, and LACS2-OEs leaves after submergence treatment for the indicated times. All of the experiments were performed for three times with similar results. The data are means ± SD (n = 6 technical replicates). Asterisks indicate significant differences from WT (** P < 0.01 by Student’s t-test). “H” and “L” indicate values that are significantly higher or lower, respectively, compared to that of WT.