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. 2020 Feb 5;9(2):193. doi: 10.3390/plants9020193

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Freezing tolerance of wild-type and transgenic Arabidopsis plantlets. (a) Phenotype of wild-type and AmDHN154, AmDHN132, and AmDHN200 transgenic plants under normal conditions (left); and their responses to freezing stress (right). (b) Survival rate after recovery from freezing (−5 °C). (c) Electrolyte leakage rate after recovery from freezing (−5 °C). AmDHN154 transformed lines: 2-10-2, 2-10-39, and 2-11-2. AmDHN132 transformed lines: 4-10-19, 4-10-30, and 4-8-18. AmDHN200 transformed lines: 7-4-29, 7-5-47, and 7-6-59. Each column is the mean of three independent experiments and bars represent the standard error of the mean. The different letters denote significant differences (p < 0.05) according to ANOVA results.

Freezing tolerance of wild-type and transgenic Arabidopsis plantlets. (a) Phenotype of wild-type and AmDHN154, AmDHN132, and AmDHN200 transgenic plants under normal conditions (left); and their responses to freezing stress (right). (b) Survival rate after recovery from freezing (−5 °C). (c) Electrolyte leakage rate after recovery from freezing (−5 °C). AmDHN154 transformed lines: 2-10-2, 2-10-39, and 2-11-2. AmDHN132 transformed lines: 4-10-19, 4-10-30, and 4-8-18. AmDHN200 transformed lines: 7-4-29, 7-5-47, and 7-6-59. Each column is the mean of three independent experiments and bars represent the standard error of the mean. The different letters denote significant differences (p < 0.05) according to ANOVA results.