Figure 2.

Sequence alignment and phylogenetic analysis of Fe²⁺/2-oxoglutarate-dependent dioxygenases involved in flavonoid biosynthesis. (A) Phylogenetic tree constructed with aligned protein sequences with MEGA 5 using the neighbor-joining method with 1000 bootstrap replications. Different Fe²⁺/2-oxoglutarate-dependent dioxygenases are grouped in clusters according to their main demonstrated activities. (B) Comparison of MnFNSI proteins with other FNSI and F3H proteins. Red arrow indicates sites determining catalytic activity of FNSI. Asterisks and black dots indicate conserved amino acids necessary for cofactor or substrate binding, respectively.