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. 2020 Feb 7;9(2):215. doi: 10.3390/plants9020215

Figure 3.

Figure 3

Functional characterization of MnFNSI. (A) UPLC chromatograms of products from MnFNSI and MnF3H with naringenin and eriodictyol as substrates. C: bovine serum albumin; A: apigenin; L: luteolin; N: naringenin; E: eriodictyol; DHK: dihydrokaempferol; DHQ: dihydroquercetin. (B) Phenotypes of transgenic tobacco harboring MnFNSI and MnF3H. (C) Detection of transgene expression by semi-quantitative RT-PCR analysis. NtActin was used as internal control gene. (D) Anthocyanin contents in control and transgenic tobacco flowers. (E) Flavone contents in flowers of control and transgenic tobacco plants.