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. 2020 Feb 24;12(2):139. doi: 10.3390/toxins12020139

Table 3.

ESI–MS/MS parameters used for the analysis of deoxynivalenol (DON), deoxynivalenol-3-glucoside (DON-3-glucoside), deoxynivalenol-3-glucuronide/deoxynivalenol-15-glucuronide (DON-3-glucuronide/DON-15-glucuronide), 3-acetyldeoxynivalenol/15-acetyldeoxynivalenol (3-ADON/15ADON), de-epoxy-deoxynivalenol (DOM-1) and isotope-labelled (13C15) deoxynivalenol (13C15 DON) in urine and the respective limits of detection and quantification.

Mycotoxin Precursor ion
(m/z)
Product ions a
(m/z)
CE a,b (eV) CV c (V) Retention time
(min)
LOD d (ng/mL) LOQ e (ng/mL)
DON 297.0 249.0/231.0 9/9 40 3.99 0.2 0.4
DON-3-glucoside 459.1 168.1/132.0 10/9 15 3.90 0.3 0.6
DON-3-glucuronide/
DON-15-glucuronide
471.0 113.0/193.0 30/24 60 3.65/3.78 0.5 1.0
3-ADON/15-ADON 339.0 231.0/203.1 15/9 15 5.69 0.1 0.2
DOM-1 281.1 215.1/233.1 9/9 40 4.83 0.6 1.2
13C15 DON 311.9 262.9/130.5 10/10 30 3.99

a Values are given as quantifier ion/qualifier ion; b CE: collision energy; c CV: cone voltage; d LOD: limit of detection; e LOQ: limit of quantification.