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. 2020 Jan 29;12(2):90. doi: 10.3390/toxins12020090

Figure 4.

Figure 4

Effect of T-2 toxin on autophagy in chicken hepatocytes. (A) The mRNA levels of Beclin-1, Atg5 and Atg7 in hepatocytes from chickens treated with T-2 toxin of different concentration at 0, 0.5, 1 and 2 mg/kg. (B) The protein expression levels of LC3, p62 and Beclin-1 in hepatocytes from chickens treated with T-2 toxin of different concentration at 0, 0.5, 1 and 2 mg/kg. (C) Morphological observation of autophagy in hepatocytes from chickens treated with T-2 toxin of different concentration at 0, 0.5, 1 and 2 mg/kg, autophagic vacuoles (red arrows, magnification from left to right: ×1200, ×5000). All the data are presented as means ± SEM of three independent experiments. * p < 0.05 and ** p < 0.01, compared with the control group.