Table 1.
Gene | Forward Primer | Reverse Primer | NCBI Accession no | Tm/℃ | Product/bp |
---|---|---|---|---|---|
Bcl-2 | 5-ATCGTCGCCTTCTTCGAGTT-3 | 5-ATCCCATCCTCCGTTGTCCT-3 | NM_205339.2 | 61 | 78 |
Atg5 | 5-GATGAAATAACTGAAAGGGAAGC-3 | 5-TGAAGATCAAAGAGCAAACCAA-3 | NM_001006409.1 | 52 | 124 |
Atg7 | 5-TCAGATTCAAGCACTTCAGA-3 | 5-GAGGAGATACAACCACAGAG-3 | NM_001030592.1 | 55 | 62 |
Beclin-1 | 5-CAGACACGCTGCTGGACC-3 | 5-TCTCCTTGTCATCCTCGTTCA-3 | NM_001006332.1 | 60 | 84 |
β-actin | 5-CCGCTCTATGAAGGCTACGC-3 | 5-CTCTCGGCTGTGGTGGTGAA-3 | NM_204313.1 | 60 | 127 |