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Lectin purification chromatogram. The 60–80% ammonium sulphate fraction was applied to the Sepharose-mannose column (1 × 11 cm), equilibrated with PBS buffer, pH 7.4. After the buffer change to 0.2 M lactose in PBS buffer, pH 7.4, at 40 mL, lectin was eluted. Fractions (2 mL) of proteins were collected and monitored for protein content at 280 nm.
