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. 2020 Feb 10;12(2):110. doi: 10.3390/toxins12020110

Table 3.

Study of BoNT-A treatment for IC/BPS in the animal.

Reference Animal Numbers Models Dose of BoNT-A Outcomes
Lucioni [50] 18 male Sprague-Dawley rats (300–350 g) Intraperitoneal injection with CYP or saline for 10-day Harvested bladders were incubated in 10 U BoNT-A for 1 h

  • Neuropeptides SP in saline group vs. CYP group: 1060 vs. 605 pg/g (p < 0.005)

  • SP in CYP group: before BTX vs. after BTX: 1060 vs. 709 pg/g (p < 0.05)
Smith [52] 21 female Sprague– Dawley rats (200–250 g) Intravesical instillation and intraperitoneal injection into four groups (n = 5–6 per group):
  • (1)

    Control (intravesical saline/intraperitoneal saline)

  • (2)

    BoNT-A (intravesical BoNT-A/intraperitoneal saline)

  • (3)

    CYP (intravesical saline/intraperitoneal CYP)

  • (4)

    CYP + BoNT-A (intravesical BoNT-A/intraperitoneal CYP)

 Bladder was instilled with 1 mL of 20 U BoNT-A for 30 min

  • BoNT-A instillation markedly reduced bladder hyperactivity induced by CYP by reducing non-voiding contraction frequency by 91%.
Cayan [51] 41 female Sprague-Dawley rats (200–300 g) Intravesical instillation of HCl (0.2 mL of 0.4 N HCl) induced chemical cystitis 2–3 U (0.2–0.3 mL) BoNT-A was injected into the detrusor at the 3, 6, 9 and 12 o’clock positions (10–12 sites)

  • Increases in the maximum bladder capacity and compliance were significantly higher in the BoNT-A group compared to the control group (p = 0.000 and p = 0.025).
Vemulakonda [53] 24 female Sprague-Dawley rats (200–250 g) Intravesical instillation and intraperitoneal injection into four groups:
  • (1)

    Saline (intravesical saline/intraperitoneal saline)

  • (2)

    BoNT-A (intravesical BoNT-A/intraperitoneal saline)

  • (3)

    CYP (intravesical saline/intraperitoneal CYP)

  • (4)

    CYP/BoNT-A (intravesical BoNT-A/intraperitoneal CYP)

 Bladder was instilled with 20 U BoNT-A for 30 min

  • After CYP treated, expression of c-fos increased significantly in L6 and S1 (78% and 107%) compared to saline control (p < 0.001).

  • Compared to the CYP group, it showed a significant decrease of c-fos expression in L6 and S1 (50% and 52%) in the BoNT-A/CYP group (p < 0.001).

  • Compared to CYP group, the increase of nonvoiding intercontractile interval was more than 10-fold in BoNT-A/CYP group (p < 0.01).

Cystitis of rats was induced by chronic CYP model that reported by Vizzard [54]. Intraperitoneal injection with CYP (150 mg/kg) was administered every third day to a total of three doses to achieve chronic inflammation. CYP: cyclophosphamide. HCl: hydrochloric acid. SP: substance P.