Fig. 4.

Effect of CMCs on cell toxicity of α-syn aggregates. a-d MTT cell viability analysis. The viability of BE(2)-M17 human cells treated with either α-syn aged alone or with a CMC1, b CMC7, c CMC110, and d CMC11 was tested by MTT assay. Cells were treated with the indicated concentrations of α-syn and CMCs for 48 h prior to the addition of MTT. The results are expressed as percentages of the average of the control (i.e., untreated cells). Means ± standard deviations are from the average of 3 wells. Statistical analysis was performed using a two-tailed unpaired t-test. ***, p < 0.001; **, p < 0.01; *, p < 0.05. e Immunocytochemistry against α-syn (green) and DAPI (blue) in BE(2)-M17 cells treated with aggregated α-syn. The cells were either non-treated or treated for 48 h with 5 μM of α-syn aged alone or in the presence of CMC1, CMC7, CMC10, and CMC11at a molar ratio of 1:4. Scale bar 30 μm