Fig. 3.

Western blot analysis of cell cycle-associated proteins. Total proteins were prepared after BGC823 cells were treated with germacrone at different concentreations (0, 20, 40, 60, and 80 μM) and then analyzed by western blotting. a Representative blotting bend intensities used for the quantification of (b) protein cyclin B1, (c) protein cdc 2 and (d) protein cdc 25c. β-actin was used as an internal control. “*”: P < 0.05, “**”: P < 0.01