Fig. 4.

Effect of germacrone on the induction of apoptosis in BGC823 cells. BGC823 cells were treated with germacrone for 24 h at different concentreations (0, 20, 40, 60, and 80 μM). a Neclear staining of BGC823 cells with Hoechst 33258. Cells were observed under fluorescence microscopy at 1000x magnification. b Annexin V-PI double staining assay detecting cell apoptosis. Percentages of early apoptotic cells (Annexin V staining) and necrotic apoptotic cells (both Annexin V-FITC and PI stainning) are shown