Fig. 6.

Effect of germacrone on the expression of apoptosis-associated proteins procaspase-3, − 6, − 8, − 9, cleaved caspase-3, − 6, − 8, − 9, and cleaved PARP. Total proteins were prepared after BGC823 cells were treated with germacrone at different concentreations (0, 20, 40, 60, and 80 μM) and then analyzed by western blotting. a Representative blotting bend intensities of procaspase-3, − 6, − 8, − 9, cleaved caspase-3, − 6, − 8, − 9 and cleaved PARP that were used for quantification of protein procaspase-8 (b), protein procaspase-9 (c), protein procaspase-3 (d) protein procaspase-6 (e), and protein cleaved PARP (f) expressions. (g) Caspase-3 activity. β-actin was used as an internal control. “*”: P < 0.05, “**”: P < 0.01