Table 1.
One Month Study Histopathology Analysis: Test of Group Differences (n = 46; 22 females, 24 males)
| Control (n = 12) |
2014 HP (n = 11) |
2016 HP (n = 11) |
2016 Butanolic HP (n = 12) |
X2 (df) p | ||
|---|---|---|---|---|---|---|
| Scoring: |
None - # (%) Mild - # (%) |
None - # (%) Mild - # (%) |
None - # (%) Mild - # (%) |
None - # (%) Mild - # (%) |
||
| Lung | Inflammation |
12 (100) 0 (0) |
11 (100) 0 (0) |
11 (100) 0 (0) |
12 (100) 0 (0) |
* |
| Edema |
12 (100) 0 (0) |
11 (100) 0 (0) |
11 (100) 0 (0) |
12 (100) 0 (0) |
* | |
| Liver | Degeneration |
12 (100) 0 (0) |
11 (100) 0 (0) |
11 (100) 0 (0) |
12 (100) 0 (0) |
* |
| Inflammation |
12 (100) 0 (0) |
11 (100) 0 (0) |
11 (100) 0 (0) |
12 (100) 0 (0) |
* | |
| Necrosis & hemorrhage |
12 (100) 0 (0) |
10 (91) 1 (9) |
11 (100) 0 (0) |
11 (92) 1 (8) |
2.10 (3) .55 | |
| Fibrosis |
12 (100) 0 (0) |
11 (100) 0 (0) |
11 (100) 0 (0) |
12 (100) 0 (0) |
* | |
| Apoptosis | ** |
** 1 |
** 1 |
** | ** | |
| Kidney | Glomerulonephritis |
12 (100) 0 (0) |
11 (100) 0 (0) |
11 (100) 0 (0) |
12 (100) 0 (0) |
* |
| Tubular degeneration / necrosis |
12 (100) 0 (0) |
9 (82) 2 (18) |
11 (100) 0 (0) |
11 (92) 1 (8) |
4.12 (3) .25 | |
| Interstitial nephritis |
12 (100) 0 (0) |
10 (91) 1 (9) |
11 (100) 0 (0) |
11 (92) 1 (8) |
2.10 (3) .55 | |
| Proteinuria |
10 (83) 2 (17) |
6 (55) 5 (45) |
8 (73) 3 (27) |
7 (58) 5 (42) |
2.80 (3) .42 *** |
|
| Heart | Inflammation |
9 (75) 3 (25) |
11 (100) 0 (0) |
11 (100) 0 (0) |
12 (100) 0 (0) |
9.09 (3) .03 |
| Necrosis |
12 (100) 0 (0) |
11 (100) 0 (0) |
11 (100) 0 (0) |
12 (100) 0 (0) |
* | |
| Fibrosis |
12 (100) 0 (0) |
11 (100) 0 (0) |
11 (100) 0 (0) |
12 (100) 0 (0) |
* | |
| Stomach | Inflammation |
12 (100) 0 (0) |
11 (100) 0 (0) |
11 (100) 0 (0) |
12 (100) 0 (0) |
* |
| Mucosal erosion / ulceration |
12 (100) 0 (0) |
11 (100) 0 (0) |
11 (100) 0 (0) |
12 (100) 0 (0) |
* | |
| Small Intestine | Inflammation |
12 (100) 0 (0) |
11 (100) 0 (0) |
11 (100) 0 (0) |
12 (100) 0 (0) |
* |
| Mucosal erosion / ulceration |
12 (100) 0 (0) |
11 (100) 0 (0) |
11 (100) 0 (0) |
12 (100) 0 (0) |
* | |
| Large Intestine | Inflammation |
12 (100) 0 (0) |
11 (100) 0 (0) |
11 (100) 0 (0) |
12 (100) 0 (0) |
* |
| Mucosal erosion / ulceration |
12 (100) 0 (0) |
11 (100) 0 (0) |
11 (100) 0 (0) |
12 (100) 0 (0) |
* | |
# Data included in the attached Excel file
* Chi-square test statistic of differences in level of response between groups not computed due to distribution of responses - no variability in level of response
** Liver apoptosis was not recorded for any male animals; only noted as “mild” in 2 female rats; no coding for the other female rats and all the male rats
*** An additional statistical test comparing control and 2014 HP groups. X2 (df) p → 2.25 (1) .13. No significant difference between the two most extreme groups