Figure 2. Characterizing the downstream responses of Aft1 induction.

1. Heatmap summary of Aft1 induction experiment showing all genes that change with |log₂(fold change)| > 1.5.
2. Parametric summaries of representative sigmoidal activation and inhibition timecourses and impulse (double sigmoid) modeling of transitory inhibition. Sigmoids are summarized by half‐max time (t _rise) and the asymptote (v _inter), while impulses include a second half‐max (t _fall) time and final asymptote (v _final). The strongest supported model for each timecourse is shown as a filled in line, while the alternative model is shown with a dashed line.
3. K‐mers enriched in the promoters of regulated genes are overlaid on summary of each gene's t _rise and v _inter. The presence of the Aft1 motif is associated with early activation, while early inhibition is associated with the PAC motif (Tod6/Dot6).
4. Response kinetics are overlaid on gene coordinates based on genetic interaction as a surrogate for functional similarity. Up‐regulated genes are enriched for vesicle trafficking/glycosylation/polarity processes, and down‐regulated genes are enriched for mitochondrial/mitotic/rRNA processes.