Fig. 3.

Indirubin treatment improves glucose tolerance and insulin sensitivity in HFD-induced obese mice. a Blood concentrations of glucose in mice fasted 16 h after 6-week treatment with either vehicle or indirubin. b Blood concentrations of insulin in mice fasted overnight after 8-week treatment with either vehicle or indirubin. c Glucose tolerance tests (GTT) performed in mice (i.p. injection of glucose, 1.5 g /kg) fasted 16 h after 6-week treatment with indirubin or vehicle. (n = 6 for each treatment). d Area under curve (AUC) for glucose based on data in c. e Insulin tolerance tests (ITT) performed in mice (i.p. injection of insulin, 0.75 U /kg) fasted 4 h after 7-week treatment with indirubin or vehicle. f Area under curve (AUC) for glucose based on data in e. Data are presented as mean ± SD (n = 6 in each group). ^#p < 0.05, ^##p < 0.01, ^###p < 0.001 compared with NCD + vehicle group; ^*p < 0.05, ^**p < 0.01, ^***p < 0.01 compared with HFD + vehicle group