Table 1.
Primary and secondary antibodies
| Epitope | Antibody | Concentration | Species |
|---|---|---|---|
| Type 1 muscle fibres | BA-D5 (DSHB) | 1 in 100 | Mouse IgG2b |
| Type IIA muscle fibres | SC-71 (DSHB) | 1 in 100 | Mouse IgG1 |
| Type IIB muscle fibres | BF-F3 (DSHB) | 1 in 100 | Mouse IgM |
| Laminin | L9393 (Sigma-Aldrich) | 1 in 100 | Rabbit |
| Mouse IgG2b | AlexaFluor 488 (Life Sciences) | 1 in 500 | Goat |
| Mouse IgG1 | AlexaFluor 647 (Life Sciences) | 1 in 500 | Goat |
| Mouse IgM | AlexaFluor 568 (Life Sciences) | 1 in 500 | Goat |
| Rabbit IgG | AlexaFluor 410 (Life Sciences) | 1 in 500 | Goat |
| Mouse IgG | AlexaFluor 488 (Life Sciences) | 1 in 250 | Goat |
| 2H3 | DSHB (Supernatant) | 1 in 50 | Mouse IgG1 |
| SV2 | DSHB (Supernatant) | 1 in 100 | Mouse IgG1 |
| Tetramethylrhodamine-bungaratoxin (−BTX, Cambridge Bioscience BT00012) | 1.5 μg/ml | ||
| NF200 | N0142, SIGMA | 1 in 500 | Mouse IgG |
| Peripherin | Merck Millipore, AB1530 | 1 in 500 | Rabbit IgG |
| VSV-G | 8G5F11, Kerafast | 1 in 2000 | Mouse IgG |
DSHB Developmental Studies Hybridoma Bank