Table 3.
The absorbance of type B trichothecenes in individual standard solutions.
| Mycotoxin | Solvent | Conc., μg/mL | λmax/λref., nm | A (Average, n = 3) | CV, % | ||
|---|---|---|---|---|---|---|---|
| Fresh | 10 Months | 14 Months | |||||
| NIV | MeOH | 20 | 217/300 | 2.36 | 2.41(+2%) | 2.42(+3%) | 1.3 |
| ACN | 50 | 217/300 | 0.28 | 0.31(+11%) | 0.35(+25%) | 11.2 | |
| DON | MeOH | 50 | 218/260 | 0.76 | 0.82(+8%) | 0.81(+7%) | 4.0 |
| ACN | 50 | 218/260 | 0.99 | 4.17 | 2.52 | 62.1 | |
| 3-AcDON | MeOH | 50 | 217/300 | 2.85 | 2.74(−4%) | 2.86(+0.4%) | 2.4 |
| ACN | 50 | 217/300 | 1.05 | 1.07(+2%) | 0.95(−10%) | 6.3 | |
| 15-AcDON | MeOH | 50 | 221/300 | 1.08 | 1.13(+5%) | 1.16(+7%) | 3.6 |
| ACN | 50 | 219/300 | 1.04 | 1.05(+1%) | 1.07(+3%) | 1.5 | |
| FusX | MeOH | 50 | 216/300 | 1.22 | 1.23(+1%) | 1.25(+2%) | 1.2 |
| ACN | 50 | 218/300 | 1.09 | 1.11(+2%) | -- | 1.3 | |
MeOH—methanol, ACN—acetonitrile, NIV—nivalenol, DON—deoxynivalenol, 3-Ac, 15-AcDON - 3-acetyl-, 15-acetyl deoxynivalenol, FusX—fusarenone X; CV—coefficient of variation of absorbance.