Table 5.
Mycotoxin | Solvent | Conc., μg/mL | λmax/λref., nm | A (Average, n = 3) | CV, % | ||
---|---|---|---|---|---|---|---|
Fresh | 10 Months | 14 Months | |||||
AOH | MeOH | 20 | 256/380 | 3.23 | 3.21 | 3.19 | |
300/380 | 0.81 | 0.81 | 0.82 | 0.7 | |||
340/380 | 0.82 | 0.81 | 0.82 | ||||
AME | ACN | 10 | 256/380 | 2.71 | 2.73 | 2.77 | |
300/380 | 0.57 | 0.57 | 0.60 | 3.0 | |||
340/380 | 0.62 | 0.62 | 0.64 | ||||
ALT | MeOH | 20 | 241/380 | 2.89 | in 4 months*: 2.90 | n.a. | |
279/380 | 1.02 | 1.03 | n.a. | 0.7 | |||
320/380 | 0.57 | 0.57 | n.a | ||||
TE | MeOH | 20 | 206/350 | 2.13 | 2.29 | 2.27 | |
282/350 | 1.32 | 1.32 | 1.35 | 1.3 |
MeOH—methanol, ACN—acetonitrile, AOH—alternariol, AME—alternariol methyl ether, ALT—altenuene, TE—tentoxin; CV—coefficient of variation of absorbance. *—The total storage period for ALT was four months, thus spectra were obtained immediately after preparation and in four months.