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The primers used for PCR
| Gene name | Primer (5′ to 3′) | Primer sequence |
|---|---|---|
| U6 | Forward | CTCGCTTCGGCAGCACA |
| Reverse | AACGCTTCACGAATTTGCGT | |
| Runx2 | Forward | CGAATAACAGCACGCTATTAA |
| Reverse | GTCGCCAAACAGATTCATCCA | |
| ALP | Forward | TAGTGAAGAGACCCAGGCGCT |
| Reverse | ATAGGCCTCCTGAAAGCCGA | |
| Col I | Forward | CCAGAA GAACTGGTACATCAGCAA |
| Reverse | CGCCATACT CGAACTGGAATC | |
| GAPDH | Forward | TCATGGGTGTGAACCATGAGAA |
| Reverse | GGCATGGACTGTGGTCATGAG | |
| Smad5 | Forward | CGGTAGCCAACTGACTTTGAGT |
| Reverse | ACCTTGTTTCCAGCCCAACA |
